human il-9 elisa kit csb-e04642h  (Cusabio)

Journal: Central-European Journal of Immunology

Article Title: Targeting the serum marker interleukin 9 improves the underlying characterization and immune homeostasis in rheumatoid arthritis

doi: 10.5114/ceji.2024.141695

Figure Lengend Snippet: Detection of serum marker levels in rheumatoid arthritis (RA) patients with different antibody types. A ) ELISA for the determination of IL-9, Gal1, anti-CII, sSR-A, anti-Sa, RF, and ACPA levels in serum of clinical samples. * p < 0.05 vs. the normal group, # p < 0.05 vs. the RA + RF negative group, & p < 0.05 vs. the RA + ACPA negative group. B ) Spearman’s analysis of the correlation of the above serum markers. The descriptive statistics are presented as mean ±SD. * p < 0.05, ** p < 0.01, **** p < 0.0001, ns. represents no significance. n = 4 in normal group, n = 10 in RA + RF negative group, n = 12 in RA + ACPA negative group, n = 13 in SNRA group

Article Snippet: We measured the levels of serum markers using ELISA with a specialized commercial human IL-9 ELISA Kit (CSB-E04642h, Cusabio, Wuhan, China), human Gal1 ELISA Kit (CSB-EL012882h, Cusabio), human anti-CII ELISA Kit (HB3371-Hu, Shanghai Hengyuan Biotechnology Co., Ltd, Shanghai, China), human sSR-A ELISA Kit (HB3370-Hu, Shanghai Hengyuan Biotechnology Co., Ltd), human anti-Sa ELISA Kit (ml382511V), human RF ELISA Kit (ml060678V), human ACPA ELISA Kit (ml233305V), rat IL-9 ELISA Kit (CSB-E07294r, Cusabio), rat Gal1 ELISA Kit (JL25762, Shanghai Jianglai Biotechnology Co., Ltd, Shanghai, China), rat anti-CII ELISA Kit (HB1364-Ra, Shanghai Hengyuan Biotechnology Co., Ltd), rat sSR-A ELISA Kit (HB1363-Ra, Shanghai Hengyuan Biotechnology Co., Ltd), rat anti-Sa ELISA Kit (ml202241V), rat RF ELISA Kit (ml059541V), and rat ACPA ELISA Kit (ml201458V).

Techniques: Marker, Enzyme-linked Immunosorbent Assay

Journal: Central-European Journal of Immunology

Article Title: Targeting the serum marker interleukin 9 improves the underlying characterization and immune homeostasis in rheumatoid arthritis

doi: 10.5114/ceji.2024.141695

Figure Lengend Snippet: Validation of serum marker expression in CIA rats. A ) Rat paw volume. B ) Rat arthritis score. C ) H&E staining was used to assess histopathologic damage in joints. D ) ELISA for serum markers (IL-9, Gal1, anti-CII, sSR-A, anti-a, RF, ACPA) in rats. The descriptive statistics are presented as mean ±SD. * p < 0.05, ** p < 0.01, n = 3 E ) Pearson analysis of the correlation between IL-9 and arthritis scores. The descriptive statistics are presented as mean ±SD. * p < 0.05, ** p < 0.01, n = 3

Article Snippet: We measured the levels of serum markers using ELISA with a specialized commercial human IL-9 ELISA Kit (CSB-E04642h, Cusabio, Wuhan, China), human Gal1 ELISA Kit (CSB-EL012882h, Cusabio), human anti-CII ELISA Kit (HB3371-Hu, Shanghai Hengyuan Biotechnology Co., Ltd, Shanghai, China), human sSR-A ELISA Kit (HB3370-Hu, Shanghai Hengyuan Biotechnology Co., Ltd), human anti-Sa ELISA Kit (ml382511V), human RF ELISA Kit (ml060678V), human ACPA ELISA Kit (ml233305V), rat IL-9 ELISA Kit (CSB-E07294r, Cusabio), rat Gal1 ELISA Kit (JL25762, Shanghai Jianglai Biotechnology Co., Ltd, Shanghai, China), rat anti-CII ELISA Kit (HB1364-Ra, Shanghai Hengyuan Biotechnology Co., Ltd), rat sSR-A ELISA Kit (HB1363-Ra, Shanghai Hengyuan Biotechnology Co., Ltd), rat anti-Sa ELISA Kit (ml202241V), rat RF ELISA Kit (ml059541V), and rat ACPA ELISA Kit (ml201458V).

Techniques: Biomarker Discovery, Marker, Expressing, Staining, Enzyme-linked Immunosorbent Assay

Journal: Central-European Journal of Immunology

Article Title: Targeting the serum marker interleukin 9 improves the underlying characterization and immune homeostasis in rheumatoid arthritis

doi: 10.5114/ceji.2024.141695

Figure Lengend Snippet: Interleukin 9 affected the severity of arthritis in CIA rats. A ) ELISA for the detection of IL-9 levels. B ) Rat arthritis score. C ) Analysis of cartilage damage by saffron O-solid green staining. The descriptive statistics are presented as mean ±SD. * p < 0.05 vs. the CIA group, n = 3

Article Snippet: We measured the levels of serum markers using ELISA with a specialized commercial human IL-9 ELISA Kit (CSB-E04642h, Cusabio, Wuhan, China), human Gal1 ELISA Kit (CSB-EL012882h, Cusabio), human anti-CII ELISA Kit (HB3371-Hu, Shanghai Hengyuan Biotechnology Co., Ltd, Shanghai, China), human sSR-A ELISA Kit (HB3370-Hu, Shanghai Hengyuan Biotechnology Co., Ltd), human anti-Sa ELISA Kit (ml382511V), human RF ELISA Kit (ml060678V), human ACPA ELISA Kit (ml233305V), rat IL-9 ELISA Kit (CSB-E07294r, Cusabio), rat Gal1 ELISA Kit (JL25762, Shanghai Jianglai Biotechnology Co., Ltd, Shanghai, China), rat anti-CII ELISA Kit (HB1364-Ra, Shanghai Hengyuan Biotechnology Co., Ltd), rat sSR-A ELISA Kit (HB1363-Ra, Shanghai Hengyuan Biotechnology Co., Ltd), rat anti-Sa ELISA Kit (ml202241V), rat RF ELISA Kit (ml059541V), and rat ACPA ELISA Kit (ml201458V).

Techniques: Enzyme-linked Immunosorbent Assay, Staining

Journal: Central-European Journal of Immunology

Article Title: Targeting the serum marker interleukin 9 improves the underlying characterization and immune homeostasis in rheumatoid arthritis

doi: 10.5114/ceji.2024.141695

Figure Lengend Snippet: Targeted inhibition of IL-9 improves inflammation and Treg/Th17 immune balance in CIA rats. A ) Proportion of Treg and Th17 cells infiltrated in arthritic tissues by flow cytometry assay. B ) Change in Treg/Th17 ratio. C ) RT-qPCR detection of foxp3 and IL-17A expression in arthritic tissues. D ) Determination of TNF-α, IL-1β, IL-6, and IL-17A levels in the synovial serum of CIA rats by ELISA. The descriptive statistics are presented as mean ±SD. * p < 0.05 vs. the CIA group, n = 3

Article Snippet: We measured the levels of serum markers using ELISA with a specialized commercial human IL-9 ELISA Kit (CSB-E04642h, Cusabio, Wuhan, China), human Gal1 ELISA Kit (CSB-EL012882h, Cusabio), human anti-CII ELISA Kit (HB3371-Hu, Shanghai Hengyuan Biotechnology Co., Ltd, Shanghai, China), human sSR-A ELISA Kit (HB3370-Hu, Shanghai Hengyuan Biotechnology Co., Ltd), human anti-Sa ELISA Kit (ml382511V), human RF ELISA Kit (ml060678V), human ACPA ELISA Kit (ml233305V), rat IL-9 ELISA Kit (CSB-E07294r, Cusabio), rat Gal1 ELISA Kit (JL25762, Shanghai Jianglai Biotechnology Co., Ltd, Shanghai, China), rat anti-CII ELISA Kit (HB1364-Ra, Shanghai Hengyuan Biotechnology Co., Ltd), rat sSR-A ELISA Kit (HB1363-Ra, Shanghai Hengyuan Biotechnology Co., Ltd), rat anti-Sa ELISA Kit (ml202241V), rat RF ELISA Kit (ml059541V), and rat ACPA ELISA Kit (ml201458V).

Techniques: Inhibition, Flow Cytometry, Quantitative RT-PCR, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Central-European Journal of Immunology

Article Title: Targeting the serum marker interleukin 9 improves the underlying characterization and immune homeostasis in rheumatoid arthritis

doi: 10.5114/ceji.2024.141695

Figure Lengend Snippet: Targeted inhibition of IL-9 regulates M2 and M1 macrophage activation. A ) Immunohistochemical detection of M1 (CD68, iNOS) and M2 (CD68, CD206) macrophage expression in arthritic tissues B ) Detection of TNF-α, IL-1β, IL-10 levels in arthritic tissues by ELISA. The descriptive statistics are presented as mean ±SD. * p < 0.05 vs. the CIA group, n = 3

Article Snippet: We measured the levels of serum markers using ELISA with a specialized commercial human IL-9 ELISA Kit (CSB-E04642h, Cusabio, Wuhan, China), human Gal1 ELISA Kit (CSB-EL012882h, Cusabio), human anti-CII ELISA Kit (HB3371-Hu, Shanghai Hengyuan Biotechnology Co., Ltd, Shanghai, China), human sSR-A ELISA Kit (HB3370-Hu, Shanghai Hengyuan Biotechnology Co., Ltd), human anti-Sa ELISA Kit (ml382511V), human RF ELISA Kit (ml060678V), human ACPA ELISA Kit (ml233305V), rat IL-9 ELISA Kit (CSB-E07294r, Cusabio), rat Gal1 ELISA Kit (JL25762, Shanghai Jianglai Biotechnology Co., Ltd, Shanghai, China), rat anti-CII ELISA Kit (HB1364-Ra, Shanghai Hengyuan Biotechnology Co., Ltd), rat sSR-A ELISA Kit (HB1363-Ra, Shanghai Hengyuan Biotechnology Co., Ltd), rat anti-Sa ELISA Kit (ml202241V), rat RF ELISA Kit (ml059541V), and rat ACPA ELISA Kit (ml201458V).

Techniques: Inhibition, Activation Assay, Immunohistochemical staining, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Central-European journal of immunology

Article Title: Targeting the serum marker interleukin 9 improves the underlying characterization and immune homeostasis in rheumatoid arthritis.

doi: 10.5114/ceji.2024.141695

Figure Lengend Snippet: Fig. 3. Interleukin 9 affected the severity of arthritis in CIA rats. A) ELISA for the detection of IL-9 levels. B) Rat arthritis score. C) Analysis of cartilage damage by saffron O-solid green staining. The descriptive statistics are pre- sented as mean ±SD. *p < 0.05 vs. the CIA group, n = 3

Article Snippet: We measured the levels of serum markers using ELISA with a specialized commercial human IL-9 ELISA Kit (CSB-E04642h, Cusabio, Wuhan, China), human Gal1 ELISA Kit (CSB-EL012882h, Cusabio), human anti-CII ELISA Kit (HB3371-Hu, Shanghai Hengyuan Biotechnology Co., Ltd, Shanghai, China), human sSR-A ELISA Kit (HB3370-Hu, Shanghai Hengyuan Biotechnology Co., Ltd), human anti-Sa ELISA Kit (ml382511V), human RF ELISA Kit (ml060678V), human ACPA ELISA Kit (ml233305V), rat IL-9 ELISA Kit (CSB-E07294r, Cusabio), rat Gal1 ELISA Kit (JL25762, Shanghai Jianglai Biotechnology Co., Ltd, Shanghai, China), rat anti-CII ELISA Kit (HB1364-Ra, Shanghai Hengyuan Biotechnology Co., Ltd), rat sSR-A ELISA Kit (HB1363-Ra, Shanghai Hengyuan Biotechnology Co., Ltd), rat anti-Sa ELISA Kit (ml202241V), rat RF ELISA Kit (ml059541V), and rat ACPA ELISA Kit (ml201458V).

Techniques: Enzyme-linked Immunosorbent Assay, Staining

Journal: Cell & Bioscience

Article Title: Tumor-derived interleukin-1 receptor antagonist exhibits immunosuppressive functions and promotes pancreatic cancer

doi: 10.1186/s13578-023-01090-8

Figure Lengend Snippet: Increased expression of interleukin-1 receptor antagonist is associated with malignant progression of PDA. (A) Left: Comparison of IL1RN mRNA level between PDA (T) and healthy pancreas (N). Expression data were analyzed from the PAAD dataset of The Cancer Genome Atlas (TCGA) (n = 182) and the normal pancreas dataset of GTExPortal (n = 328). TPM: transcript per million. Student’s t-test was performed. Right: Kaplan-Meier survival analysis between two groups of patients by a cut-off value of IL1RN mRNA expression (low IL1RN vs. high IL1RN). FPKM: fragments per kilobase of exons per million mapped fragments. Data were analyzed by Human Protein Atlas website. (B) Correlation analyses between IL1RN mRNA level and ten gene programs (GP1-GP10), which were assigned into four subtypes of PDA. Each gene program consists of a different number of signature genes (labeled in parenthesis). Correlation coefficients are visualized by a heat map. (C) Immunohistochemical (IHC) analysis using an antibody against IL-1Ra protein (gene name: IL1RN ) (brown). Patient-derived xenografts (PDX#1-#2) were derived from patients with PDA. Scale: 30 μm. (D) IHC analyses of IL-1Ra (brown) and FOXP3 (green) using clinical tissues directly from a PDA patient (upper panel). An identical procedure was performed in tumor-adjacent normal pancreas from the same patient, showing epithelial cells of a pancreatic duct (arrow) (lower panel). Scale: 60 μm. (E) Monitoring human serum IL-1Ra levels by ELISA. Sera were collected from PDA patients (Tumor, n = 17) and healthy donors (Normal, n = 20) at Taipei Medical University Hospital (Taipei, Taiwan). Student’s t-test was performed. (F) Correlation analysis of serum IL-1Ra and CA19-9 in the PDA patients’ sera (n = 17). r : Pearson’s correlation coefficient. * p < 0.05. ** p < 0.01. **** p < 0.0001

Article Snippet: Mouse and human IL-1Ra/IL-1F3 (Quantikine ELISA Kit; R&D Systems, Minneapolis, MN, USA), mouse IL-1β ELISA kits (Abcam, Cambridge, UK), and human cancer antigen CA19-9 ELISA kit (Abcam) were used to detect specific cytokine secretions.

Techniques: Expressing, Labeling, Immunohistochemical staining, Derivative Assay, Enzyme-linked Immunosorbent Assay

Journal: Diagnostics

Article Title: New Challenges in the Diagnosis of Kidney Damage Due to Immune Checkpoint Inhibitors Therapy: An Observational Clinical Study

doi: 10.3390/diagnostics13152524

Figure Lengend Snippet: Study design outline. Objectives based on treatment progression. GM2AP: GM2 ganglioside activator protein; NAG: N-acetyl-beta-D-glucosaminidase; KIM: kidney injury molecule-1; NGAL: neutrophil gelatinase-associated lipocalin; IL-9: Interleukin 9, TNF-α: tumour necrosis factor alpha.

Article Snippet: In addition, two inflammatory biomarkers that can help in this differential diagnosis will be evaluated: Interleukin 9 (IL-9) (Invitrogen IL-9 Human ELISA Kit, Waltham, MA, USA) and tumour necrosis factor alpha (TNF-α) (Invitrogen TNF alpha Human Instant ELISA™ Kit, Waltham, MA, USA).

Techniques: